DocumentCode :
2498497
Title :
Analysis of Relationship between Apoptosis and Changes of Ca2+ in HepG-2 Induced by CSA with Laser Scanning Confocal Technology
Author :
Ji Yu bin ; Yu Lei ; Zhang Xiao min
Author_Institution :
Center of R&D on Life Sci. & Environ. Sci., Harbin Univ. of Commerce, Harbin, China
fYear :
2009
fDate :
11-13 June 2009
Firstpage :
1
Lastpage :
4
Abstract :
Laser scanning confocal technology was used to study relationship between apoptosis and changes of Ca2+ induced by CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effects of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation on HepG-2 cells was completed by fluorescence microscope. Apoptosis of HepG-2 cells induced by CSA was measured by flowcytometry. In addition, change of intracellular Ca2+ level of CSA on HepG-2 cells was observed by laser scanning confocal microscope. CSA had obvious cytotoxicity on HepG-2 in a dose-dependent manner, and its IC50 was 162.4 mug/ml. CSA could induce characteristic apoptosic morphology of HepG-2 cells, and apoptosis percentage was significantly higher than control one. Migration of cells cycle from S phase to G2 phase had been blocked by CSA. Concentration of Ca2+ in HepG-2 had been increased by CSA, which was positive correlation with drug dosage. CSA had obvious effects of killing and inducing apoptosis on human heptocarcinoma cell HepG-2, and overload of Ca2+ might be involved in the events.
Keywords :
biomedical measurement; biomedical optical imaging; cancer; cell motility; dosimetry; drugs; fluorescence; laser applications in medicine; optical microscopy; tumours; CSA killing effect; HepG-2 cell; MTT method; cell cycle migration; correlation method; dose-dependent manner; drug dosage; flowcytometry measurement; fluorescence microscope; human heptocarcinoma cell; laser scanning confocal microscope; Business; Drugs; Educational technology; Fluorescence; Humans; Laser theory; Laser transitions; Microscopy; Probes; Research and development;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
Type :
conf
DOI :
10.1109/ICBBE.2009.5162353
Filename :
5162353
Link To Document :
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