Title :
cDNA Cloning and Bioinformatic Analysis of Self-Incompatible S34-Allele from Chinese Pears
Author :
Zeng, Yanling ; Tan, Xiaofeng ; Zhang, Dangquan ; Hu, Xiaoyi ; Chen, Hongpeng ; Yuan, Deyi ; Zhang, Lin
Author_Institution :
Non-wood Forest Products of State Forestry Adm., Central South Univ. of Forestry & Technol., Changsha, China
Abstract :
The full-length cDNA of a new S-RNase (S 34-RNase) (GenBank accession No.DQ414813) was successfully cloned from pistils of Chinese pear by RT-PCR and RACE methods. The S 34-RNase shows a complete CDS of 687 nucleotides for putative 229 amino acids. The theoretical three-dimension structure of S 34-RNase was predicted by homology modeling method and its structural characteristic was analyzed. Aligning of S 34-RNase with other 39 S-RNases of pears revealed the question about unifying naming system.
Keywords :
DNA; bioinformatics; botany; genetics; molecular biophysics; Chinese pear pistils; RACE method; RT-PCR method; S34-RNase 3D structure; amino acid; bioinformatic analysis; cDNA cloning; full-length cDNA; homology modeling method; nucleotides; self-incompatible S34-allele; unifying naming system; Amino acids; Bioinformatics; Biological materials; Cloning; Computer aided analysis; Forestry; Nitrogen; Plants (biology); Predictive models; RNA;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162479
