Title :
cDNA Microarray Image Processing Using Morphological Operator and Edge-Enhancing Diffusion
Author_Institution :
Sch. of Mechanic & Electron. Eng., Soochow Univ., Suzhou, China
Abstract :
Complementary DNA (cDNA) microarray imaging technology is a revolutionary innovation for genomic research that allows monitoring of expression levels for thousands of genes simultaneously. The raw data of cDNA microarray is shown as red and green channel images that are quantitatively analysed to obtain the gene expression measurements. It can affect the subsequent analysis such as identification of genes differentially expressed. cDNA microarray image analysis includes three tasks: image filtering, segmentation and information extraction. This paper presents a novel-filtering framework that is capable of processing cDNA microarray images. The experimental result is compared with those obtained from the widely used Gaussian de-noising techniques, wavelet de-noising techniques and morphological filtering technique. Simulation in this paper indicates that the proposed edge-enhancing diffusion filter and morphological operators are computationally attractive, yield excellent performance and are able to preserve structural information while efficiently suppressing noise in cDNA microarray data. The result of experiment shows that it is robust and precise.
Keywords :
biodiffusion; biological techniques; biology computing; edge detection; feature extraction; genomics; image denoising; image enhancement; image segmentation; lab-on-a-chip; wavelet transforms; Gaussian denoising technique; cDNA microarray image processing; edge-enhancing diffusion; gene expression measurement; genomic research; green channel image; image filtering; image segmentation; information extraction; morphological operator filter; red channel image; wavelet de-noising technique; Bioinformatics; DNA; Genomics; Image analysis; Image processing; Information filtering; Information filters; Monitoring; Noise reduction; Technological innovation;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162481