Cloning and Bioinformatics Analysis of Genomic DNA Sequences of Gastrodia Tuber

Gastrodia tuber has many pharmacological effects. DNA fingerprints of Gastrodia tuber were determined using randomly amplified polymorphic DNA (RAPD) technique, of which the desired DNA segments were selected and recovered. Positive DNA clones were obtained through ligation techniques, transformation, blue/white color screenings, cloning and identifying. Novelty of the cloned DNA and its potential importance were confirmed by sequencing DNA and bioinformatics analyzing. The results showed the DNA sequences were proven to be a new discovery, Sequence 1 and 3 had 3 and 1 Open Reading Frames (ORF), respectively. DNA sequence 2 have 13 ORFs, which can express 8 unintegrated function domains. The DNA sequence deserve further researching. The distribution of these 3 DNA sequences varied greatly among the populations, whereby DNA sequence 1 exist only in the populations that show the highest content of effective component gastrodin. The findings may be applied in exploiting genomic DNA and genetic classification of Gastrodia tuber populations.