Title :
Detection of Viable Listeria monocytogenes in Dairy Products By Real Time Reverse-Transcription PCR
Author :
Yan, Bing ; Zhao, Feng ; Bi, Yuhan ; Xiang, Li ; Jiang, Yujun
Author_Institution :
Key Lab. of Dairy Sci., Northeast Agric. Univ., Harbin, China
Abstract :
Listeria monocytogenes is a food-borne pathogen which can cause zoonosis. A detection method based on real time reverse-transcription PCR amplification of mRNA was established in the study. This method can overcome false-positive result caused by amplification of nonviable L.monocytogenes. Sensitivity and specificity of the method were studied, as well as artificially contaminated dairy products by L.monocytogenes, dairy products from market and L.monocytogenes biofilm. For contaminated milk, detection sensitivity was 17CFU/ml after 6 h enrichment. Detection limit in biofilm was 2times102CFU/cm2 after 7 h enrichment. The results indicate that the method can satisfy detection of L.monocytogenes in dairy products and biofilm.
Keywords :
biological techniques; contamination; enzymes; food safety; microorganisms; organic compounds; Listeria monocytogenes; PCR; biofilm; dairy products; food-borne pathogen; mRNA; reverse-transcription; zoonosis; Bismuth; Capacitive sensors; DNA; Dairy products; Food technology; Laboratories; Microorganisms; Pathogens; Probes; RNA;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162613