Determination of BSA by Its Enhancement Effect on Second Order Scattering of Chrome Azurol S

Second order scattering (SOS) used to be regarded as a kind of interfere of spectrofluorometry. The characteristic and principle of SOS have not been studied deeply. In this paper SOS was found to be a new method to determine trace substance with good potential of analysis. The reaction between Chrome Azurol S (CAS) and bovine serum albumin (BSA) was investigated by the method of second order scattering. It was found the SOS intensity of CAS and that of BSA is weak respectively in the BR (Britton-Robinson) buffer solution at PH 4.10 with the presence of SDS micelle, but sharply enhanced when the two compounded, based on which a new method for determination of BSA was established, which has high sensitivity and calls for simple operation. The concentration of BSA is linear with CAS in the range of 1.0~2.0 mugldrmL-1. The new method was applied to determination of BSA in real samples with satisfactory results.