Title :
Drug Release Properties of Poly (D, L-Lactic Acid) PDLLA Modified Genipin Cross-Linked Chitosan/Gelatin Scaffold
Author :
Tian, Jun-Sheng ; Cui, Yuan-Lu ; Yao, Kang-De
Author_Institution :
Key Lab. of Pharmacology of Traditional Chinese Med. Formulae, Tianjin Univ. of Traditional Chinese Med., Tianjin, China
Abstract :
In the present study, poly (D, L-lactic acid) (PDLLA) modified genipin-cross-linked chitosan/gelatin scaffolds loaded icariin were prepared. The scaffolds with genipin cross-linking were fabricated with lyophilization, and then were immersed into the 2.5%, 5.0%, and 7.5% PDLLA-dichloromethane solutions. Thus the PDLLA modified genipin-cross-linked scaffolds were obtained after evaporating the solvent dichloromethane. The water absorption capacity and degradation rate of the scaffolds were measured and evaluated. The released icariin was determined with High Performance Liquid Chromatography (HPLC) method. The swelling ratio and degradation rate decreased significantly after the PDLLA modification. The loaded icariin released gradually in 9 days reaching to the maximum of 6.137, 4.530, 3.745, and 4.13 mg/g, respectively. The XTT assay was applied to evaluate the cytotoxicity and biocompatibility of the scaffolds with MC3T3-E1 osteoblast-like cells in this study.
Keywords :
biochemistry; biodegradable materials; biomedical materials; drug delivery systems; gelatin; polymers; swelling; tissue engineering; MC3T3-E1 osteoblast-like cells; PDLLA modified genipin; PDLLA-dichloromethane solution; XTT assay; biocompatibility; chitosan; cytotoxicity; degradation rate; drug release properties; gelatin scaffold; genipin cross-linking; high performance liquid chromatography; icariin; lyophilization; poly(D,L-lactic acid); swelling ratio; water absorption capacity; Absorption; Biological materials; Chemicals; Drugs; Extracellular; Implants; Laboratories; Polymer gels; Solvents; Thermal degradation;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162664
