Title :
Dynamic Monitoring of PEG-Mediated Cell Fusion by RT-CES System
Author :
Deng, Huihua ; Guo, Airong ; Qian, Mengdie ; Liu, Xuan ; Zhou, Dingxin ; Tang, Zuming ; Lu, Zuhong
Author_Institution :
Key Lab. of Child Dev. & Learning Sci. (SEU), Southeast Univ., Nanjing, China
Abstract :
The response of MCF7 and A549 cells to the PEG treatment was monitored in the terms of impedance-related cell index curves by RT-CES system. The treatment of 50% PEG to MCF7 and A549 cells led to the one-stage drop of cell index with the culture time. Longer duration of PEG action to cells shortened the net detachment duration of cells from the surface of the electrode. The treatment of 15% PEG to MCF cells resulted in two-stage drop of cell index. These were attributed to PEG-mediated cell fusion, which was supported by SEM results.
Keywords :
bioelectric phenomena; biomedical measurement; biosensors; cellular biophysics; electric impedance; scanning electron microscopy; A549 cell response; MCF7 cell response; PEG-mediated cell fusion; RT-CES system; SEM; cell detachment duration; cell-based biosensor; dynamic monitoring; impedance-related cell index curves; real-time cell electronic sensing system; Biology; Biosensors; Cells (biology); Electric variables measurement; Electrodes; Impedance measurement; Monitoring; Real time systems; Sensor arrays; Surface morphology;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162668
