Title :
Effect of Asparagus Polysaccharide on the Number and Activity of Erythrocyte Complement Receptor 1(CD35) of S180 Mice
Author :
Chen Feng Ji ; Ji, Chen Feng ; Min, Yong Cui ; Chen, Xue Jun
Author_Institution :
Eng. Res. Center of Natural Antineoplastic Drugs, Harbin Univ. of Commerce, Harbin, China
Abstract :
To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1(CD35) in S180 mice. Red blood cells from mice venous blood are labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of CR1. Using microscope, we studied the adherence between erythrocyte immunity and C3b receptor or tumor-cell by RBC-C3bRR and DTER. Comparing the mean value of the number of CR1 on erythrocyte of high and middle groups with control groups, the mean value of the number of CR1, RBC-C3bRR and DTER of Asparagus officinalis polysaccharide groups are increased significantly. Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice, which may be one of antitumor mechanisms.
Keywords :
adhesion; blood; cancer; cellular biophysics; drugs; proteins; tumours; Asparagus officinalis polysaccharide effect; CD35 monoclonal antibody; DTER; FITC-conjugated goat antimouse antibody; RBC-C3bRR; adhesion; drugs; erythrocyte complement receptor 1; erythrocyte immunity; flow cytometry; mice venous blood; red blood cells; tumor-cell; Abdomen; Animals; Blood; Business; Drugs; Immune system; Mice; Microscopy; Neoplasms; Testing;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162685
