Effect of N-Terminal and C-Terminal Deletion of Cry1Ie of Bacillus thuringiensis on Its Expression and Purification

Author

Shen, Huicong ; Zhang, Chunlu ; Guo, Shuyuan ; Zhang, Jie ; Song, Fuping

Author_Institution

Sch. of Life Sci. & Technol., Beijing Inst. of Technol., Beijing, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

4

Abstract

Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1le genes were silent in Bt strains, but can be over expressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1le protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.

Keywords

biochemistry; cellular biophysics; genetics; microorganisms; molecular configurations; proteins; Bacillus thuringiensis; Bt strain; C-terminal amino acid; Escherichia coli BL21; N-terminal amino acid; Ostrinia furnacalis; Plutella xylostella; cry1le gene; crystal protein structure formation; gram-positive bacterium; soybean pod borer; sporulation; Amino acids; Biology; Capacitive sensors; Diseases; Insects; Laboratories; Plants (biology); Protection; Proteins; Purification;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5162705

Filename

5162705