Title :
Effect of N-Terminal and C-Terminal Deletion of Cry1Ie of Bacillus thuringiensis on Its Expression and Purification
Author :
Shen, Huicong ; Zhang, Chunlu ; Guo, Shuyuan ; Zhang, Jie ; Song, Fuping
Author_Institution :
Sch. of Life Sci. & Technol., Beijing Inst. of Technol., Beijing, China
Abstract :
Crystal proteins are produced by the gram-positive bacterium Bacillus thuringiensis (Bt) during sporulation. The cry1le genes were silent in Bt strains, but can be over expressed in Escherichia coli BL21 (DE3) in inclusion bodies, and the expressed product was toxic to Plutella xylostella, Ostrinia furnacalis, and soybean pod borer (Leguminivora glycinivorella). In order to know the N-terminal and C-terminal amino acid on the structure formation of Cry1le protein, four deletion mutants were constructed and the expression and purification of these mutants were studied.
Keywords :
biochemistry; cellular biophysics; genetics; microorganisms; molecular configurations; proteins; Bacillus thuringiensis; Bt strain; C-terminal amino acid; Escherichia coli BL21; N-terminal amino acid; Ostrinia furnacalis; Plutella xylostella; cry1le gene; crystal protein structure formation; gram-positive bacterium; soybean pod borer; sporulation; Amino acids; Biology; Capacitive sensors; Diseases; Insects; Laboratories; Plants (biology); Protection; Proteins; Purification;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162705
