Title :
Effect of Wastewater Ultraviolet Disinfection of Power Ultrasonic Enhancement
Author :
Zhang, Shenghua ; Wu, Chundu
Author_Institution :
Sch. of Environ., Jiangsu Univ., Zhenjiang, China
Abstract :
Power ultrasound (US) has been employed to enhance the effect of ultraviolet (UV) disinfection, and the mechanism was investigated based on molecular biology. The experiment result showed that there was a good linear correlation between the UV irradiation dose and inactivation rate of E.Coli. By orthogonal test, ultrasound power, action time and volume of bacterial liquid had no statistical significance to inactivation rate, and the greatest factor was the volume of bacterial liquid, then the ultrasound power, and then the action time. The optimal conditions found were: volume 50 mL; power 600 W; time 20 S. Under the optimal operating point that 200 W US for 20 S, the inactivation rate was 0.22 log units in 200 mL bacterial liquid. The inactivation rate in the synergistic system between the optimal operating condition and UV was bigger than which in the independent effect of ultrasound plus UV, which demonstrated that the ultrasound pretreatment under optimal operating point can strengthen the effect of UV disinfection. The electronic microscope photograph and the gel electrophoretic map indicated that the ultrasound could not destroy the DNA but only change the cell structure. This study also utilized the specific recognition to the thymine dimmer of T4 endonuclease V to investigate the mechanism of the optimal condition.
Keywords :
biological effects of ultraviolet radiation; biomolecular effects of radiation; decontamination; electrophoresis; enzymes; microorganisms; ultrasonic applications; wastewater treatment; E.Coli inactivation; T4 endonuclease V thymine dimmer; UV irradiation dose; bacterial liquid volume; cell structure change; electronic microscope photograph; gel electrophoretic map; molecular biology; orthogonal test; power 600 W; power ultrasonic enhancement; synergistic system; time 20 s; wastewater ultraviolet disinfection effect; Biochemistry; Capacitive sensors; DNA; Electron microscopy; Lamps; Microorganisms; Temperature control; Testing; Ultrasonic imaging; Wastewater;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162720