Effects of Water and Chloroform Extracts of Chinese Medicinal Insects on Polysaccharides Production by Ganoderma sinense in Submerged Fermentation

Water and chloroform extracts of various Chinese medicinal insects were added into the medium of Ganoderma sinense to investigate the effects of the extracts on production of biomass, intracellular polysaccharides (IPS), and extracellular polysaccharides (EPS). The results showed that chloroform extract of E. sinensis (CES) at 55 mg/L lead to significant increase in both biomass and IPS concentration from 7.29 plusmn 0.51 to 10.36 plusmn 0.39 g/L and 591.1 plusmn 29.1 to 622.3 plusmn 24.6 mg/L, respectively. In addition, chloroform extract of E. sinensis at 35 mg/L and water extract of C. molossus at 55 mg/L showed stimulatory effects on EPS production, and their EPS yield significantly enhanced from 365.2 plusmn 18.7 to 421.8 plusmn 16.1 mg/L and 365.2 plusmn 19.1 to 413.4 plusmn 17.1 mg/L, respectively. Both the extracts can be used as easily available stimulators for the biomass or polysaccharide production by submerged fermentation of G. sinense.