DocumentCode :
2508439
Title :
Expression of Caspase-3 Gene in Gastric Adenocarcinoma Cell Line SGC-7901 via Ad-FasL
Author :
Zheng Shi-ying ; Ge Jin-feng ; Zhao Jun ; Jiang Dong ; Li Hong
Author_Institution :
Dept. of Thoracocardiac Surg., First Affiliated Hosp. of Suzhou Univ., Suzhou, China
fYear :
2009
fDate :
11-13 June 2009
Firstpage :
1
Lastpage :
5
Abstract :
The objective of this study is to investigate the relationship between caspase-3 expression and apoptosis in the gastric adenocarcinoma cell line SGC-7901 transfected with the adenovirus-mediated FasL gene. The human gastric adenocarcinoma cell line SGC-7901 was transfected with the adenovirus-mediated FasL gene. The expression of caspase-3 and apoptotic morphological changes were measured by flow cytometry, TUNEL, RT-PCR, and immunocytochemical assays. The expression of caspase-3 was low in the gastric adenocarcinoma cell line SGC-7901, but transfection of these cells with Ad-FasL enhanced the cellular expression of the caspase-3 gene and induced susceptibility to Fas/FasL-mediated apoptosis (25.4% vs. 7.2%, p < 0.001). Apoptosis exhibited a positive relationship with caspase-3 expression (gamma = 0.647, p < 0.01). The down-regulation of caspase-3 expression and resistance to Fas/FasL-mediated apoptosis in gastric adenocarcinoma cells were found to be related to the development and progression of the cells. FasL gene transfer may increase caspase-3 expression in gastric adenocarcinoma cells and enhance apoptosis in them.
Keywords :
biological organs; biomedical measurement; biomembranes; cancer; cellular biophysics; enzymes; genetics; microorganisms; molecular biophysics; proteins; tumours; FasL gene transfer; adenovirus-mediated FasL gene; apoptotic morphological change; caspase-3 gene expression; cell death; flow cytometry measurement; gastric adenocarcinoma cell; immunocytochemical assay; membrane glycoprotein; Birth disorders; Cancer; Cells (biology); Hospitals; Immune system; Packaging; Polymers; RNA; Sequences; Surgery;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
Type :
conf
DOI :
10.1109/ICBBE.2009.5162833
Filename :
5162833
Link To Document :
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