A study on the construction of microbial consortia containing bacterial isolates capable of degrading AB113, ABK 24, MB17

The present work focuses on the Microbial degradation of three textile azo dyes, Acid Blue 113(AB113), Acid Black 24(ABK24) and Mordant Black 17 (MB17) by thirteen bacterial isolates as well by their consortia. Thirty five bacterial isolates were isolated from textile dyeing industry effluents. Proficient and rapid dye degradation was identified in thirteen isolates. Microbial consortia were developed on the basis of dye degrading competence of the strains. Nearly 60%-95% degradation was achieved in less than about 72 h in these isolates tested on individual dyes. Promising results were obtained with mixed dye concentration. The dye concentration, pH, temperature and time of degradation were optimized. 16S rRNA gene amplification was carried out for the sequencing and identification of these strains. TLC, HPLC, FTIR and GC-MS analysis were carried out to confirm the presence of aromatic amines and to aid the data supporting the course of degradation. As identified from the analyses, the final products of AB113 degradation were Metanilic acid and Peri acid, the latter was seen to disappear after 72h of degradation.