Title :
Improving the Stability of Cellulase by Immobilization on Chitosan-Coated Magnetic Nanoparticles Modified alpha-Ketoglutaric Acid
Author :
Zhou, Yu-Ting ; Su, Sai-Nan ; Song, Ming-Min ; Nie, Hua-Li ; Zhu, Li-Min ; Branford-White, Christopher J.
Author_Institution :
Coll. of Chem., Chem. Eng. & Biotechnol., Donghua Univ., Shanghai, China
Abstract :
The application of cellulases in various industries demands highly stable enzymes, able to perform at extreme pH values and temperatures. In this study, the cellulase, which was immobilized on the chitosan-coated magnetic nanoparticles modified with alpha-ketoglutaric acid (alpha-KA-CCMNPs), exhibited a broader pH range of high activity and the loss of the activity of immobilized cellulase was lower than that of free cellulase. And the optimal temperature for immobilized cellulase was 50degC, whereas that for free cellulase was 40degC. Meanwhile, the immobilized cellulase showed better thermal stability than free cellulase at high temperature range. In addition, the storage stability of the cellulase was improved greatly after it was immobilized on the surface of alpha-KA-CCMNPs.
Keywords :
biothermics; enzymes; magnetic particles; molecular biophysics; nanoparticles; pH; thermal stability; alpha-KA-CCMNP; alpha-ketoglutaric acid; cellulase stability; chitosan-coated magnetic nanoparticle; pH value; stable enzyme; storage stability; temperature 40 degC; temperature 50 degC; thermal stability; Biochemistry; Chemicals; Magnetic materials; Magnetic separation; Nanoparticles; Stability; Sugar; Surface resistance; Temperature; Textile industry;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162941
