Title :
Isolation, Identification, and Antimicrobial Activity of a Denitrifying Bacterium from Marine Sediments
Author :
Fu, Bufei ; Zhang, Yi ; Mu, Jun ; Zhao, Youbao ; Zhu, Xiuhua
Author_Institution :
Sch. of Environ. & Chem. Eng., Dalian Jiaotong Univ., Dalian, China
Abstract :
A strain of denitrifying bacterium DN7, a facultative aerobic bacterim, was isolated from the sea area near the Changhai County of Dalian. Some physiological and biochemical properties were characterized, 16S rDNA gene sequence was amplified by PCR, and its nucleotide sequence was determined. Based on its physiological and biochemical properties, homology and phylogenetic analysis, strain DN7 was identified as a subspecies of Pseudomonas stutzeri. In addition, its antimicrobial activity assay and the culture conditions´ effect on its biomass and activity were also studied and reported here.
Keywords :
DNA; biochemistry; cellular biophysics; genomics; microorganisms; oceanography; sediments; 16S rDNA gene sequence; Changhai county; Dalian; PCR; Pseudomonas stutzeri subspecies; antimicrobial activity assay; denitrifying bacterium antimicrobial activity; denitrifying bacterium identification; denitrifying bacterium isolation; facultative aerobic bacterium; marine sediments; nucleotide sequence; strain DN7 bacteria; strain DN7 biochemical properties; strain DN7 homology analysis; strain DN7 phylogenetic analysis; strain DN7 physiological properties; Capacitive sensors; Methanol; Microorganisms; Oceanographic techniques; Phylogeny; Sampling methods; Sediments; Sequences; Sugar; Testing;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5162998
