Kinetic Analysis of Aminoethylisothiourea on Diphenolase of Mushroom Tyrosinase

Author

Li, Shu-Bai ; Zhang, Hai-Tao ; Xue, Yong ; Nie, Hua-Li ; Zhu, Li-Min ; Zhang, Xiao-Fei ; Branford-White, Christopher

Author_Institution

Coll. of Chem., Chem. Eng. & Biotechnol., Donghua Univ., Shanghai, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

4

Abstract

This study demonstrates that Aminoethylisothiourea (AET), a potent inhibitor of inducible nitric oxide synthase (NOS), is an irreversible competitive inhibitor of the diphenolase activity of mushroom tyrosinase when _L-DOPA was assayed spectrophotometrically in vitro. The inhibition of diphenolase activity of tyrosinase by AET showed positive kinetic cooperativity which arose from the protection of both substrate and o-quinone against inhibition by AET.

Keywords

biochemistry; enzymes; molecular biophysics; reaction kinetics; spectrophotometry; aminoethylisothiourea; diphenolase; inducible nitric oxide synthase; inhibitor; kinetic analysis; mushroom tyrosinase; o-quinone; spectrophotometry; Biochemistry; Copper; Data analysis; Drugs; Inhibitors; Kinetic theory; Nonlinear equations; Proteins; Switches; Variable structure systems;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5163003

Filename

5163003