Lipase Catalyzed Synthesis of Ethyl Lactate

Author

Huang Zhihong ; Gao Jing ; Zhao Tiantao ; Li Weijie ; Zhou Liya ; He Ying

Author_Institution

Sch. of Chem. Eng. & Technol., Hebei Univ. of Technol., Tianjin, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

4

Abstract

Ethyl lactate was synthesized by lipase catalyzed between lactic acid and ethanol in organic medium for the first time. The experiments showed that the optimal solvent is Tert-buty alcohol and the optimal lipase is Novozym 435. The optimal reaction with a conversion of 77% can be reached when the molar ratio (acid: alcohol) is 1:8, the temperature is 60degC, the acid concentration is 0.3 mol/L, the lipase concentration is 45 g/mol, reaction time is 24 hours and the revolutions is 200 rpm. Novozym 435 can be used continuously for six times and a conversion of 60% can be reached.

Keywords

biochemistry; biotechnology; catalysis; enzymes; solvents (industrial); Novozym 435 lipase; ethanol reagent; ethyl lactate lipase catalysed synthesis; lactic acid reagent; organic medium; temperature 60 degC; tert-buty alcohol solvent; time 24 hour; Biochemistry; Chemical analysis; Chemical technology; Environmentally friendly manufacturing techniques; Ethanol; Molecular sieves; Pollution; Silicon compounds; Solvents; Temperature;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5163016

Filename

5163016