Long Term Sensibility of the Ni-Co Protein Chip

For the metal ion affinity chromatography, the nickel is used to capture the histidines(his)-tagged protein to make up a rapid purification system from the organism lysate. In this study, based on the optimization approach of Taguchi method, we developed a Ni-Co protein chip whose intermediate metal surface could capture the recombinant protein successfully. Thus, the proposed Ni-Co chip can provide specific binding with His-tagged biotin and His-tagged Uricase. In addition, under the constant humidity and temperature, the immobilization capability of Ni-Co chip with specific binding of immuno- reaction was tested every week. Finally, we obtained the long term performance of Ni-Co chip with 6timesHis-Uricase of concentration of 20 mug/ml.