Abstract :
The purpose of the present study was to use Capacitive Coupling of pulsed electromagnetic field (CC-PEMF) and hydroxyapatite nanoparticles (HAp) as a countermeasure to prevent osteoporosis induced by simulated microgravity. We have used the hind limb-suspended (HLS) rat model to simulate microgravity induced bone losses for 45 days and in order to assess resulting Mineralogical (BMD, Ca and P), Biochemical (osteocalcin, ALP and Type I collagen) and histological (SEM) changes and to study the effect of countermeasure (PEMF and HAp). Three-month-old, female wistar rats were randomly divided into controls (n =8), Hindlimb suspended (HLS) n =8), HLS with pluse electromagnetic field (HLS+PEMF) n=8), HLS with hydroxyapatite nanoparticles (HLS+HAp, n =8), and HLS with HAp and PEMF (HLS+PEMF+HAp, n=8). We observed: 1). a significant decrease (p<0.01) in BMD, Calcium, Phosphorus, Collagen I and ALP activity in Femur and Tibia in hind limb bone and serum osteocalcin in HLS rat as compared to ground control.2) Non-significant increase in BMD (P<0.1), calcium (P<0.1), phosphorus(P<0.5),collagen I(P<0.1) and ALP activity(P<0.5) in femur and tibia in hind-limb bone and serum osteocalcin(P<0.5) in HLS+PEMF rats as compare to HLS. While 3). Significant increase in BMD (P<0.02), Calcium (P<0.05), Phosphorus (P<0.05), Collagen I (P<0.02), and ALP activity (p<0.02) in femur and tibia in hind-limb bone and non-significant increase in serum osteocalcin (P<0.1) in HLS+HAp rat as compared to HLS. 4). Highly significant increase in BMD (P<0.01), calcium (P<0.01), phosphorus (P<0.01), collagen I (P<0.01) and ALP activity (P<0.01) in femur and tibia in hind-limb and serum osteocalcin (P<0.02). The present results suggest that a combination of low level pulsed electromagnetic field (CC-PEMF) and silicon-substituted hydroxyapatite nanoparticles (HAp) has potential to control bone loss induce due to simulated microgravi- - ty.
Keywords :
biochemistry; bioelectric phenomena; biological effects of fields; biomagnetism; bone; calcium compounds; diseases; electromagnetic fields; nanoparticles; nanotechnology; patient treatment; phosphorus compounds; proteins; silicon; ALP activity; Ca10(PO4)6(OH)2-Si; biochemical changes; bone loss; calcium; capacitive coupling; femur; hind bone; hind limb suspended rat model; histological changes; mineralogical changes; phosphorus collagen; pulsed electromagnetic field therapy; serum osteocalcin; silicon-substituted hydroxyapatite nanoparticles; simulated microgravity induced osteoporosis; tibia; time 45 d; Bones; Calcium; EMP radiation effects; Electromagnetic coupling; Electromagnetic fields; High level synthesis; Medical treatment; Nanoparticles; Osteoporosis; Rats; ALP; HAP; HLS; HLS+N; HLS+P; HLS+P+N; OC; PEMF;
