DocumentCode :
2514681
Title :
Novel Affinity Membrane Chromatography Technology: Purification of Pepsin Using Immobilized Reactive Dye Ligands
Author :
Chen, Tian-Xiang ; Li, Shu-Bai ; Nie, Hua-Li ; Zhu, Li-Min ; Branford-White, Christopher J.
Author_Institution :
Chem. Eng. & Biotechnol., Donghua Univ., Shanghai, China
fYear :
2009
fDate :
11-13 June 2009
Firstpage :
1
Lastpage :
4
Abstract :
Novel membranes were prepared through covalently coupling of chitosan (CS) to activate nylon membrane after the reaction of the microporous nylon membrane with formaldehyde. Non-specific adsorption on the CS-coated nylon membrane decreased greatly. Yellow 3G-P/Reactive Red 120 were then covalently immobilized onto composite membrane as dye ligands. These Red 120/Yellow 3G-P-CS-membranes were used in the Pepsin adsorption studies. According to the results, it appears that Yellow 3G-P-CS-nylon membranes could be applied for separation and purification of Pepsin without causing any denaturation, much better than Red 120-CS-nylon membrane.
Keywords :
biomedical materials; chromatography; dyes; membranes; polymers; purification; Pepsin purification; adsorption; affinity membrane chromatography technology; chitosan; formaldehyde; immobilized reactive dye ligands; nylon membrane; Biochemistry; Biomembranes; Chemical analysis; Chemical technology; Chemistry; Instruments; Pharmaceuticals; Purification; Scanning electron microscopy; Temperature;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
Type :
conf
DOI :
10.1109/ICBBE.2009.5163122
Filename :
5163122
Link To Document :
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