Preparation and Evaluation of Implantable Chitosan-Collagen-Soybean Phosphatidylcholine Film Impregnated with Mitomycin C-PLA- Nanoparticles

This study was designed to develop and evaluate a chitosan-collagen-soybean phosphatidylcholine (SPC) complex film impregnated with mitomycin C (MMC)-PLA- nanoparticles for treatment of hepatocellular carcinoma in mice. MMC-PLA-nanoparticles were prepared by a reverse micelle-solvent evaporation method. PLA-MMC-nanoparticles were dispersed throughout the film. SEM, ATR-FTIR were used to character the properties of the complex film. In vitro, MMC release from the MMC contained complex film (MMC-film) was measured by incubation of the film in phosphate buffered saline with drug analysis by using UV/vis spectrometer at 366 nm. The MMC-film exhibited release characteristics with 8.01% of MMC being released at Id and an accumulated drug release over 30 d was 40.32%. In vivo, the MMC-film was implanted at subcutaneous tumor sites of mice and the curative effect was compared with those of drug free complex film. The growth of the tumors were inhibited considerably and dose-dependently by MMC-film (P<0.05). In addition, the post-mortem visual examination did not show any signs of internal inflammation and infection nor were there any evidence of implant encapsulation in any of the animals after 20 d of implantation of the MMC-film or drug free complex film. The results of this work suggest that the chitosan-collagen- SPC complex film has the potential to serve as an effective delivery system for MMC.