Title :
Purification and Some Properties of Superoxide Dismutase from Aloe saponaria (Ait) Haw.
Author :
Tang Yun-Ming ; Tang Liang-ting ; Xu Rong-Min
Author_Institution :
Sch. of Life Sci., Southwest China Univ., Chongqing, China
Abstract :
Superoxide dismutase (SOD) from Aloe saponaria (Ait) Haw was purified to electrophoretic homogeneity by a sequential steps including of acetone and heat of treatment, DEAE-sepharose and Sephacryl S-200 column chromatography. Three SOD components were obtained, designated as SOD-I,SOD-II,SOD-III with the molecular weight of 46,000D, 35,000D and 40,000D, and isoelectric point of 8.9, 7.6, 7.35 respectively. The three components were consisted of two identical subunits. All three SOD components from A. saponaria (Ait) Haw were Fe-SOD as confirmed by its sensitivity to KCN and H202, elemental analyses and the absorption spectrum analyses.
Keywords :
biochemistry; chromatography; electrophoresis; heat treatment; molecular weight; organic compounds; purchasing; purification; Aloe saponaria Haw; acetone; chromatography; electrophoretic homogeneity; heat treatment; molecular weight; purification; superoxide dismutase; Absorption; Australia; Biochemistry; Cardiovascular diseases; Filtration; Heat treatment; Organisms; Proteins; Purification; Spectroscopy;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5163262
