Label free detection of biomolecular interactions

Author

Hradetzky, David ; Müller, Claas ; Reinecke, Holger

Author_Institution

Lab. for Process Technol., Freiburg Univ.

fYear

2005

fDate

1-4 Aug. 2005

Firstpage

81

Lastpage

82

Abstract

This work presents a simple evanescent wave sensing system based on an interferometric approach suitable to meet the demands on label free sensor systems for the detection of biomolecular interactions especially. It is representing a basic concept towards high throughput application. The system performance and reproducibility was evaluated with refractometric measurements of sucrose dilutions. A mean resolution of the effective refractive index of 3sigma(Deltamacrnmacr_eff)=0.9middot10^-6 without averaging and a reproducibility below sigma_r=0.1middot10^-6 were achieved. The suitability for the detection of biomolecular interactions was validated with the detection of a DNA hybridization

Keywords

DNA; biological techniques; fibre optic sensors; genetics; light interferometry; molecular biophysics; refractive index measurement; DNA hybridization detection; biomolecular interaction detection; evanescent wave sensing system; interferometric approach; label free sensor system; refractometric measurements; sucrose dilutions; Biosensors; Electromagnetic waveguides; Optical interferometry; Optical refraction; Optical sensors; Optical surface waves; Optical waveguides; Refractive index; Reproducibility of results; Sugar;

fLanguage

English

Publisher

ieee

Conference_Titel

Optical MEMS and Their Applications Conference, 2005. IEEE/LEOS International Conference on

Conference_Location

Oulu

Print_ISBN

0-7803-9278-7

Type

conf

DOI

10.1109/OMEMS.2005.1540087

Filename

1540087