Title :
Label free detection of biomolecular interactions
Author :
Hradetzky, David ; Müller, Claas ; Reinecke, Holger
Author_Institution :
Lab. for Process Technol., Freiburg Univ.
Abstract :
This work presents a simple evanescent wave sensing system based on an interferometric approach suitable to meet the demands on label free sensor systems for the detection of biomolecular interactions especially. It is representing a basic concept towards high throughput application. The system performance and reproducibility was evaluated with refractometric measurements of sucrose dilutions. A mean resolution of the effective refractive index of 3sigma(Deltamacrnmacreff)=0.9middot10-6 without averaging and a reproducibility below sigmar=0.1middot10-6 were achieved. The suitability for the detection of biomolecular interactions was validated with the detection of a DNA hybridization
Keywords :
DNA; biological techniques; fibre optic sensors; genetics; light interferometry; molecular biophysics; refractive index measurement; DNA hybridization detection; biomolecular interaction detection; evanescent wave sensing system; interferometric approach; label free sensor system; refractometric measurements; sucrose dilutions; Biosensors; Electromagnetic waveguides; Optical interferometry; Optical refraction; Optical sensors; Optical surface waves; Optical waveguides; Refractive index; Reproducibility of results; Sugar;
Conference_Titel :
Optical MEMS and Their Applications Conference, 2005. IEEE/LEOS International Conference on
Conference_Location :
Oulu
Print_ISBN :
0-7803-9278-7
DOI :
10.1109/OMEMS.2005.1540087
