Synthesis and Characterization of A Novel Natural Macromolecule Antibacterial Material

A novel natural macromolecule Antibacterial material was synthesized by reaction with carboxymethyl-chitosan, decanoyldimethylammonium and epichlorohydrin. Long chain quaternary ammonium (OGTA) was prepared by reaction with decanoyldimethylammonium and epichlorohydrin, using isopropylalcohol as solvent, at 55degC for 6 hours, and vacuum distillation. The yield was 87 percent. With 0.5 g CMCTS and 2 g OGTA as initiative materials, 0.5 g sodium hydroxide regulating the value of PH, reacting at 30 ml N-methylpyrrolidone for 8 hours and stirring quickly at 70degC, carboxymethylchitosan derivative (CMCTS-OGTA) was obtained. The yield was 95 percent. Characterization of the product by IR spectrum, ¹H-NMR spectrum and element analysis revealed that the experiments had got the wanted product. Bacteriostatic activity and antibacterial activity were characterized by the greatest bacteriostatic circle method, which indicated that CMCTS-OGTA definitely had an inhibitory activity against S. aureus and bacillus subtilis. Comparation of the Bacteriostatic activities of CMCTS-OGTA and CMCTS suggested that CMCTS-OGTA had more perfect Antibacterial capability. The lowest effectively antibacterial concentrations against Bacillus coli and S. aureus were also determined.