Title :
The cDNA Segment Cloning and Bioinformatics Analysis of SREBP-2 Gene in Goose
Author :
Han Chun-Chun ; Wang Ji-Wen ; Li Liang ; Wang Wei
Author_Institution :
Key Lab. of Animal Genetic Resources, Sichuan Agric. Univ., Ya´an, China
Abstract :
Total RNA from Goose liver of 16 weeks old was isolated. The 365 bp cDNA segment was amplified by the reverse transcription polymerase chain reaction (RT-PCR). Sequence analysis showed that there is a high similarity between the fragment of cDNA and Sterol regulatory element binding protein-2 (SREBP-2) gene. This segment contains a bHLH motif, basic region and leucine zipper domain. Meanwhile, the bHLH motif has a structure of helix-loop-helix, and the two helixes integrate and form a zipper structure.
Keywords :
DNA; bioinformatics; genetics; molecular biophysics; Sterol regulatory element binding protein-2 gene; bHLH motif; bioinformatics; cDNA segment; cloning; helix-loop-helix structure; leucine zipper domain; reverse transcription polymerase chain reaction; sequence analysis; Amino acids; Animals; Biochemistry; Bioinformatics; Cloning; Genetics; Liver; Polymers; Proteins; RNA;
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
DOI :
10.1109/ICBBE.2009.5163582
