A direct injection method for blood cells into microchannels from pure blood droplets with switchable in-situ distillation of erythrocytes

This paper represents a direct injection method for leukocytes into microfluidic channels using negative dielectrophoresis. The blood sample is a heparinated pure blood droplet and injection is realized on-chip without any external microfluidic connections to the microchip. During injection erythrocytes are immobilized in-situ at injection reservoir electrodes. The method is easily applicable to most BioMEMS applications requiring direct injection of cells from pure blood and to eliminate pressure driven control.