Title :
Molecular delivery and transfection by laser-induced oscillating microbubbles
Author :
Qihui Fan ; Wenqi Hu ; Ohta, Aaron T.
Author_Institution :
Dept. of Mech. Eng., Univ. of Hawai´i at Manoa, Honolulu, HI, USA
Abstract :
Exogenous molecules were successfully delivered into localized single mammalian adherent cells with oscillating microbubbles induced by microsecond laser pulses. The shear stress due to the oscillation of the microbubble causes the poration of a nearby cell. Specific single cells can be porated by adjusting the laser position to create the microbubbles beneath the target cell. The poration of localized single cells was demonstrated with the delivery of 500-kDa FITC-Dextran dye and 16-kb plasmids.
Keywords :
bioMEMS; cellular biophysics; molecular biophysics; shear strength; FITC-Dextran dye; exogenous molecules; laser-induced oscillating microbubbles; localized single cell poration; mammalian adherent cells; microsecond laser pulses; molecular delivery; molecular transfection; shear stress; Cells (biology); Fluorescence; Semiconductor lasers; Stress; Substrates; Ultrafast optics; cell poration; microbubbles; molecular delivery; transfection;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2014 9th IEEE International Conference on
Conference_Location :
Waikiki Beach, HI
DOI :
10.1109/NEMS.2014.6908813
