DocumentCode
252767
Title
Molecular delivery and transfection by laser-induced oscillating microbubbles
Author
Qihui Fan ; Wenqi Hu ; Ohta, Aaron T.
Author_Institution
Dept. of Mech. Eng., Univ. of Hawai´i at Manoa, Honolulu, HI, USA
fYear
2014
fDate
13-16 April 2014
Firstpage
302
Lastpage
305
Abstract
Exogenous molecules were successfully delivered into localized single mammalian adherent cells with oscillating microbubbles induced by microsecond laser pulses. The shear stress due to the oscillation of the microbubble causes the poration of a nearby cell. Specific single cells can be porated by adjusting the laser position to create the microbubbles beneath the target cell. The poration of localized single cells was demonstrated with the delivery of 500-kDa FITC-Dextran dye and 16-kb plasmids.
Keywords
bioMEMS; cellular biophysics; molecular biophysics; shear strength; FITC-Dextran dye; exogenous molecules; laser-induced oscillating microbubbles; localized single cell poration; mammalian adherent cells; microsecond laser pulses; molecular delivery; molecular transfection; shear stress; Cells (biology); Fluorescence; Semiconductor lasers; Stress; Substrates; Ultrafast optics; cell poration; microbubbles; molecular delivery; transfection;
fLanguage
English
Publisher
ieee
Conference_Titel
Nano/Micro Engineered and Molecular Systems (NEMS), 2014 9th IEEE International Conference on
Conference_Location
Waikiki Beach, HI
Type
conf
DOI
10.1109/NEMS.2014.6908813
Filename
6908813
Link To Document