Fibroblasts connection on nano/micro silicon

We propose a silver nanoparticle etching technology to fabricate nano/micro surfaces. The behaviors of HIG-82 fibroblasts can be controlled by nano/mirco patterned silicon surface in vitro. The microposts were defined through photolithography and silver nanoparticles assisted etching was used to produce the nanostuctures. After that, the hydrophobic chemical was coated on the surface of nanostructures to form hydrophilic/hydrophobic composite structures. In order to investigate cell connection between microposts, fluorescence dye was used to observe fibroblasts. According to the fluorescence pictures, the major component of connected parts is consisted of f-actin.