Title :
Localized electroporation of mouse embryo using dielectric guides
Author :
Mazari, E. ; Mantelet, M. ; Iranzo, J. ; Perea-Gomez, A. ; Gosse, C.
Author_Institution :
Lab. de Photonique et de Nanostruct., LPN-CNRS, Marcoussis, France
Abstract :
We devised, fabricated, and validated a microsystem allowing one to introduce locally exogenous molecules in mouse embryos during their early development. Relying on a set of two macroscopic electrodes and two narrow tunnels filled with buffer, as few as several tens of cells can be modified upon application of the appropriate voltage drop.
Keywords :
bioelectric phenomena; biological techniques; biomedical electrodes; biomembranes; cellular biophysics; buffer filled narrow tunnels; dielectric guides; localized mouse embryo electroporation; locally exogenous molecules; macroscopic electrodes; microsystem; voltage drop application; Computational modeling; Dielectrics; Electric potential; Electrodes; Embryo; Mice; Resists; Biocompatibility; development; microfluidic sticker; soft UV imprint; transgenesis;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International
Conference_Location :
Beijing
Print_ISBN :
978-1-4577-0157-3
DOI :
10.1109/TRANSDUCERS.2011.5969320
