DocumentCode :
2531800
Title :
Temperature modulated excitation and phase sensitive detection to selectively image DNA sequences
Author :
Zrelli, K. ; Cavatore, E. ; Barilero, T. ; Berthoumieux, H. ; Croquette, V. ; Le Saux, T. ; Jullien, L. ; Lemarchand, A. ; Gosse, C.
Author_Institution :
Dept. de Chimie, Ecole Normale Super., Paris, France
fYear :
2011
fDate :
5-9 June 2011
Firstpage :
2160
Lastpage :
2163
Abstract :
Temperature oscillations allow one to modulate the concentration of reagents and products with given amplitude and phase shift, both parameters depending on the dynamics of the chemical system under study. We here rely on thermal excitation in a microdevice, associated with fluorescence video microscopy as well as image filtering protocols, to selectively enhance the signal issued from a labeled probe that hybridizes with a targeted oligonucleotide. Furthermore, by simply tuning the stimulation parameters, focus can be put either on the perfect match sequence or on some other one that bears a point mutation.
Keywords :
DNA; biomedical optical imaging; biothermics; fluorescence; DNA sequence selective imaging; fluorescence video microscopy; oligonucleotide; phase sensitive detection; product conecntration; reagent conecntration; temperature modulated excitation; temperature oscillation; thermal excitation; Fluorescence; Heating; Microscopy; Modulation; Probes; Temperature sensors; Joule heating; Rate constant; fluorescence microscopy; kinetics; point mutation;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International
Conference_Location :
Beijing
ISSN :
Pending
Print_ISBN :
978-1-4577-0157-3
Type :
conf
DOI :
10.1109/TRANSDUCERS.2011.5969322
Filename :
5969322
Link To Document :
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