A high-throughput fully-automated microfluidic live cell array for combination drug treatment analysis of colorectal cancer cells

Author

Wang, H. ; Kim, J. ; Jayaraman, A. ; Cypert, M. ; Hua, J. ; Bittner, M. ; Han, A.

Author_Institution

Dept. of Electr. & Comput. Eng., Texas A&M Univ., College Station, TX, USA

fYear

2011

fDate

5-9 June 2011

Firstpage

2148

Lastpage

2151

Abstract

Combination chemotherapies play an important role in clinical cancer treatment. We have previously developed a microfluidic high-throughput drug screening platform and used it for the screening of prostate cancer sensitizing agents. We have further developed the system into a fully automated live cell array platform with uniform cell seeding for better control, and used the platform to investigate the gene expression regulation of colorectal cancer cells in response to combination cancer drug treatment. The results showed significant drug effects on the proliferation of the HCT116 colorectal cancer cells, demonstrating the potential of this microfluidic device as a high-throughput combination chemotherapeutic drug-screening platform.

Keywords

bioMEMS; cancer; cellular biophysics; drugs; genetics; microfluidics; HCT116 colorectal cancer cells; cancer treatment; cell proliferation; cell seeding; chemotherapeutic drug-screening platform; chemotherapies; drug treatment analysis; fully automated live cell array platform; gene expression regulation; high-throughput fully-automated microfluidic live cell array; microfluidic device; microfluidic high-throughput drug screening platform; prostate cancer sensitizing agents; Arrays; Cancer; Cancer drugs; Fluorescence; Generators; Microfluidics; High-throughput screening (HTS); colorectal cancer cell; combination drug treatment; microfluidic live cell array;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International

Conference_Location

Beijing

ISSN

Pending

Print_ISBN

978-1-4577-0157-3

Type

conf

DOI

10.1109/TRANSDUCERS.2011.5969335

Filename

5969335