Label-free real time imaging of neural communication using acetylcholine image sensor

Release of acetylcholine (ACh) by a local K⁺ ion stimulation to neurons was successfully imaged using charge transfer type label-free ACh image sensor. In the non-stimulation area, the ACh release from the neurons was observed and the released ACh gradually diffused with time. These data suggest that the images of ACh release induced by local depolarization are involved in the propagation signals mediated by the neurotransmission at the nerve terminals. This report demonstrates the prospect for the label-free neural communication imaging for the first time.