Title :
High-performance protein preconcentrator using microchannel-integrated nafion strip
Author :
Yang, H. ; Shen, M. ; Sivagnanam, V. ; Gijs, M.A.M.
Author_Institution :
Lab. of Microsyst., Ecole Polytech. Fed. de Lausanne, Lausanne, Switzerland
Abstract :
A microfluidic device for protein preconcentration based on the electrokinetic trapping principle is proposed. It comprises a Nafion strip which is simply cut from a commercially available membrane and integrated into a polydimethylsiloxane (PDMS) microfluidic structure using a guiding channel. By applying low voltage (<;15 V), the device exhibits a preconcentration factor of >;103 of fluorescently labeled bovine serum albumin (BSA) within a few minutes. Moreover, a numerical study of the preconcentration effect is presented to microscopically understand the phenomenon in our type of device.
Keywords :
electrokinetic effects; microchannel flow; proteins; bovine serum albumin; electrokinetic trapping principle; high-performance protein preconcentrator; microchannel-integrated nafion strip; microfluidic device; polydimethylsiloxane microfluidic structure; Biomembranes; Electrokinetics; Fluorescence; Microfluidics; Nanobioscience; Proteins; Strips; Nafion; Protein preconcentrator; electrokinetic trapping; microfluidics;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International
Conference_Location :
Beijing
Print_ISBN :
978-1-4577-0157-3
DOI :
10.1109/TRANSDUCERS.2011.5969416
