In-situ closed loop hydrogel membrane formation by one step stamping

The use of membranes within microfluidics has been widely studied, such as separating of components and culturing supports of scaffolds for tissue engineering. A few methods for in-situ formation of membranes have been reported. However, these methods are hard to control the width of membrane or difficult to realize stable interfacial membranes. Furthermore, these conventional methods are impossible to form the closed loop membrane intrinsically. In this study, we suggest a simple method for the in-situ formation of hydrogel membranes with various patterns and closed loop shapes. Especially, we firstly demonstrate the multi-chemotaxis device with closed loop membrane.