A double trapped single cell contact and interaction system via movable poly (ethylene glycol) diacrylate (PEG-DA) microstructure for immune analysis

Some subsets of immune cells execute their missions depending on cell contact and interaction, like natural killer cell and cytotoxic T lymphocyte. To understand the movement exhibited by cellular aggregates, we must understand how the local interactions between moving cells affect the collective motion. In this paper, we demonstrated a double trapped single cell chip by positive dielectrophoresis (pDEP) force with the movable poly (ethylene glycol) diacrylate (PEG-DA) for cell-cell contact microenvironment. In our design, such as S-shape channel, L-shaped double-lock and l-shaped double-lock, we could evaluate the NK cell activity in single cell level. Based on our data, we provided an easily-performed method for study the interaction of immune cells.