Nonthermal plasma effects on mesenchymal stem cell differentiation

Stem cells have been known to be differentiated by chemical and physical environmental changes. Non-thermal plasma was applied to the cell culture substrates. Chemical modifications induced on carbon-based materials or biocompatible polymers had effects on mesenchymal stem cell growth and differentiation. Various gases were fed in nonthermal plasma and the following chemical modifications were screened using Raman spectroscopy. The adsorption of adhesion proteins and the differentiation of mesenchymal stem cells were screened by using fluorescence microscopy, and the molecular works were followed. This result suggests easy and cost-efficient method of platform functionalization for stem cell therapy.