Title :
Chemical IC chip for dynamical control of protein synthesis
Author :
Ikuta, Koji ; Maruo, Shoji ; Fujisawa, Tetsuya ; Fukaya, Yuichiro
Author_Institution :
Sch. of Eng., Nagoya Univ., Japan
Abstract :
The several types of biochemical IC chips with photodetective micro reactors have been developed. The real-time monitoring and control of cell free synthesis of protein was demonstrated successfully. The photo protein of the firefly, the so called Luciferase, was synthesized. The biochemical luminescence was monitored by a built-in photodiode in the micro reactor to transfer reaction status into a voltage signal. Feedback control synthetic reaction was verified experimentally. The results achieved here should be key technology for future DNA and RNA synthesis without a biological cell
Keywords :
biochemistry; biocontrol; biological specimen preparation; bioluminescence; biotechnology; feedback; microactuators; microfluidics; micropumps; molecular biophysics; monolithic integrated circuits; photodetectors; process monitoring; proteins; DNA synthesis; Luciferase synthesis; RNA synthesis; biochemical IC chip; biochemical luminescence monitoring; built-in photodiode; dynamic control; feedback control; firefly; photo protein; photodetective micro reactor; protein synthesis; reaction status; voltage signal; Chemicals; Feedback control; Inductors; Integrated circuit synthesis; Luminescence; Monitoring; Photodiodes; Proteins; Signal synthesis; Voltage;
Conference_Titel :
Micromechatronics and Human Science, 1998. MHS '98. Proceedings of the 1998 International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
0-7803-5130-4
DOI :
10.1109/MHS.1998.745792
