Nonthermal bioplasma characteristics of reactive radical species´ density & electron temperature and their interactions with biological cells

We have investigated the basic plasma density and electron tempereature by using the collisional radiative model, and also investigated the reactive radical species´s densities for hydroxyl OH, superoxide anion O2*^- and nitric oxide NO by using the ultravilioet absorption spectroscopy with the deuterium lamp in the atmospheric pressure nonthermal argon bioplasma sources bombarding the water surface. It is noted especially that the plsama density and electron temperature are key parameters for the production of OH, O2*^- and NO radical species. These radical species play a very important role in the interactions between the bioplasma and biological cells. It is noted that the plasma density and electron temperature are measured to be about (1.1-1.7) eV and (0.7-5.4)×10¹² cm^-3, respectively, under Ar gas flow ranged from 80 to 300 sccm in this experiment. Also the densities for the OH, O2*^- , and NO species are measured to be (0.5-7.7)×10¹⁵ cm^-3, (0.2-4.5)×10¹⁵ cm^-3, and (0.3-8.2)×10¹⁴ cm^-3, respectively, during the nonthermal bioplasma treatments of the biological cells in this study. We have also investigated the nonthermal bioplasma interactions with microbial E. Coli, filamentous fungus Neurospora crassa, yeast Saccaromyces cerevisiae, mammalian blood cells, and T98G human brain cancer cells in morphologicl and biomolecular aspects under lethal dose for understanding the exact biological pathways caused by several reactive radical species in bioplasma. It is found from the biological cells that the molecular electron band energy structure has been shifted toward the vacuum surface energy by bioplasma interactions. Also intracellular genomic DNA content in fungal tissues has been shown to be dramatically reduced by a plasma treatment and the transcription factor tah-3 is found to be a crucial protein for fungal to- erance to a these plasma environments. We also found that apoptosis cell death and micronucleus formation rate of T98G brain cancer cells are caused by these plasma parameters.