Characterisation of Herpes Simplex Virus particles by dielectrophoreic crossover methods

Author

Hughes, Michael P. ; Morgan, Hywel ; Rixon, Frazer J.

Author_Institution

Bioelectron. Res. Centre, Glasgow Univ., UK

Volume

6

fYear

1998

fDate

29 Oct-1 Nov 1998

Firstpage

2816

Abstract

Dielectrophoresis (DEP) is a technique which has been applied to the investigation of the biophysical properties of cells and other micron-scale particles. Recent advances in electrode fabrication have enabled the application of dielectrophoretic techniques to the analysis of sub-micron particles such as latex nanospheres, proteins and viruses. In this paper the dielectrophoretic properties of the human pathogen Herpes Simplex Virus, type 1 (HSV-1) as a function of suspending medium conductivity are presented. This study confirms previous estimates of dielectric properties of the HSV-1 virion. These properties are: surface conductance 3 nS, internal relative permittivity 68, internal conductivity 7 mSm^-1

Keywords

bioelectric phenomena; biological techniques; cellular biophysics; electrophoresis; permittivity measurement; 3 nS; Herpes simplex virus particles characterisation; cellular biophysical properties; electrode fabrication advances; human pathogen; internal conductivity; internal relative permittivity; latex nanospheres; micron-scale particles; surface conductance; suspending medium conductivity; Conductivity; Dielectrics; Dielectrophoresis; Electrodes; Fabrication; Humans; Pathogens; Permittivity; Proteins; Viruses (medical);

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1998. Proceedings of the 20th Annual International Conference of the IEEE

Conference_Location

Hong Kong

ISSN

1094-687X

Print_ISBN

0-7803-5164-9

Type

conf

DOI

10.1109/IEMBS.1998.746069

Filename

746069