Title :
Characterisation of Herpes Simplex Virus particles by dielectrophoreic crossover methods
Author :
Hughes, Michael P. ; Morgan, Hywel ; Rixon, Frazer J.
Author_Institution :
Bioelectron. Res. Centre, Glasgow Univ., UK
fDate :
29 Oct-1 Nov 1998
Abstract :
Dielectrophoresis (DEP) is a technique which has been applied to the investigation of the biophysical properties of cells and other micron-scale particles. Recent advances in electrode fabrication have enabled the application of dielectrophoretic techniques to the analysis of sub-micron particles such as latex nanospheres, proteins and viruses. In this paper the dielectrophoretic properties of the human pathogen Herpes Simplex Virus, type 1 (HSV-1) as a function of suspending medium conductivity are presented. This study confirms previous estimates of dielectric properties of the HSV-1 virion. These properties are: surface conductance 3 nS, internal relative permittivity 68, internal conductivity 7 mSm-1
Keywords :
bioelectric phenomena; biological techniques; cellular biophysics; electrophoresis; permittivity measurement; 3 nS; Herpes simplex virus particles characterisation; cellular biophysical properties; electrode fabrication advances; human pathogen; internal conductivity; internal relative permittivity; latex nanospheres; micron-scale particles; surface conductance; suspending medium conductivity; Conductivity; Dielectrics; Dielectrophoresis; Electrodes; Fabrication; Humans; Pathogens; Permittivity; Proteins; Viruses (medical);
Conference_Titel :
Engineering in Medicine and Biology Society, 1998. Proceedings of the 20th Annual International Conference of the IEEE
Conference_Location :
Hong Kong
Print_ISBN :
0-7803-5164-9
DOI :
10.1109/IEMBS.1998.746069
