A novel cell-positioning technique for extracellular recording and impedance measurement on single cells using planar electrode substrates

For single-cell-biosensor applications and for studying neural networks using planar electrode substrates often the position of cells on detectors is necessary. Here, the authors present a new method for fast placement of single cells on ring electrodes by suction on suction holes. They describe the fabrication of a micromachined planar electrode substrate with suction holes and the placement of L929 cells and Neuro 2A cells on particular positions. The results are discussed regarding the deciding factors for placement. Finally, applications of the authors´ cell placement technique to form patterned neural networks are outlined