Title :
Study on activity of soluble Catechol-O-Methyltransferase in rat liver using high performance liquid chromatography
Author :
Yang, Lina ; Deng, Yulin
Author_Institution :
Sch. of Life Sci. & Technol., Beijing Inst. of Technol., Beijing, China
Abstract :
A method was used to investigated S-Catechol-O-Methyltransferase activity(COMT) with 3,4-dihydroxybenzoicacid as the substrate C18 column (150 mm ×4.6 mm) at 25°C. The mobile phase consisted of methanol and 0.07 % acetic acid aqueous solution with a flow rate of 1.0 mL/min at 254 nm. The optimal pH value was 8 and temperature was 45°C for S-COMT specific activity.
Keywords :
enzymes; 3,4-dihydroxybenzoicacid; COMT; acetic acid aqueous solution; high performance liquid chromatography; methanol; pH value; rat liver; soluble catechol-o-methyltransferase activity; substrate C18 column; temperature 25 C; temperature 45 C; Circuits; Fingers; Force sensors; Humans; Instruments; Liver; Neural networks; Prosthetic hand; Tendons; Voltage; Solublecatechol-O-methyltransferase; activity; detection; pH; temperature;
Conference_Titel :
Bioinformatics and Biomedical Technology (ICBBT), 2010 International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-6775-4
DOI :
10.1109/ICBBT.2010.5478949
