• DocumentCode
    2569675
  • Title

    Study on activity of soluble Catechol-O-Methyltransferase in rat liver using high performance liquid chromatography

  • Author

    Yang, Lina ; Deng, Yulin

  • Author_Institution
    Sch. of Life Sci. & Technol., Beijing Inst. of Technol., Beijing, China
  • fYear
    2010
  • fDate
    16-18 April 2010
  • Firstpage
    329
  • Lastpage
    331
  • Abstract
    A method was used to investigated S-Catechol-O-Methyltransferase activity(COMT) with 3,4-dihydroxybenzoicacid as the substrate C18 column (150 mm ×4.6 mm) at 25°C. The mobile phase consisted of methanol and 0.07 % acetic acid aqueous solution with a flow rate of 1.0 mL/min at 254 nm. The optimal pH value was 8 and temperature was 45°C for S-COMT specific activity.
  • Keywords
    enzymes; 3,4-dihydroxybenzoicacid; COMT; acetic acid aqueous solution; high performance liquid chromatography; methanol; pH value; rat liver; soluble catechol-o-methyltransferase activity; substrate C18 column; temperature 25 C; temperature 45 C; Circuits; Fingers; Force sensors; Humans; Instruments; Liver; Neural networks; Prosthetic hand; Tendons; Voltage; Solublecatechol-O-methyltransferase; activity; detection; pH; temperature;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Bioinformatics and Biomedical Technology (ICBBT), 2010 International Conference on
  • Conference_Location
    Chengdu
  • Print_ISBN
    978-1-4244-6775-4
  • Type

    conf

  • DOI
    10.1109/ICBBT.2010.5478949
  • Filename
    5478949
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=2569675