DocumentCode
2569677
Title
Lifetime estimation of moving vesicles in frequency-domain fluorescence lifetime imaging microscopy
Author
Roudot, Philippe ; Kervrann, Charles ; Waharte, François
Author_Institution
Inria Rennes - Bretagne Atlantique, Rennes, France
fYear
2012
fDate
2-5 May 2012
Firstpage
668
Lastpage
671
Abstract
We propose a robust statistical framework for correcting the movements of vesicles in frequency domain FLIM imaging. Movement and lifetime are jointly estimated in a three-step procedure. Robust M-estimators are mainly used to improve accuracy in temporally-varying noisy images. The performance of the proposed method is demonstrated on both simulated and real samples.
Keywords
biomechanics; biomedical optical imaging; cellular biophysics; fluorescence; microorganisms; optical microscopy; statistical analysis; frequency domain FLIM imaging; frequency-domain fluorescence lifetime imaging microscopy; lifetime estimation; robust M-estimators; robust statistical framework; temporally-varying noisy imaging; vesicles movements; Estimation; Fluorescence; Frequency domain analysis; Microscopy; Robustness; Tracking; FLIM; M-estimation; frequency-domain; phase modulation; spot tracking; template matching;
fLanguage
English
Publisher
ieee
Conference_Titel
Biomedical Imaging (ISBI), 2012 9th IEEE International Symposium on
Conference_Location
Barcelona
ISSN
1945-7928
Print_ISBN
978-1-4577-1857-1
Type
conf
DOI
10.1109/ISBI.2012.6235636
Filename
6235636
Link To Document