Title :
A modification and purify method to culture the Schwann cells by adult SD rat
Author :
Lao, J. ; Hung, L.K. ; Wong, W.S.
Author_Institution :
Dept. of Ortho. & Traum., Hong Kong Chinese Univ., Shatin, Hong Kong
fDate :
29 Oct-1 Nov 1998
Abstract :
Describes a technique of controlled enzyme digestion of peripheral nerves to obtain a highly purified suspension of Schwann cells. An average of 1.72×107 cells/ml was obtained from a 1 cm segment of ulnar nerve in the grown-up SD rat. Comparison with conventional technique and simultaneous culture of adventitial tissues from the same nerve confirmed the lack of contamination by fibrocytes
Keywords :
biological specimen preparation; cellular biophysics; neurophysiology; suspensions; 1 cm; Schwann cells culture; adventitial tissues; biological research technique; controlled enzyme digestion technique; fibrocytes contamination; modification/purify method; ulnar nerve; Biochemistry; Brachytherapy; Contamination; Hospitals; Humans; Microscopy; Pediatrics; Rodents; Roentgenium; Testing;
Conference_Titel :
Engineering in Medicine and Biology Society, 1998. Proceedings of the 20th Annual International Conference of the IEEE
Conference_Location :
Hong Kong
Print_ISBN :
0-7803-5164-9
DOI :
10.1109/IEMBS.1998.746105
