Influence of bioelectret collagen on cell growth in vitro

Author

Yang, X.L. ; Li, B.S. ; Gu, J.W. ; Sun, S. ; Zhu, H.S.

Author_Institution

Res. Centre of Mater. Sci., Beijing Inst. of Technol., China

Volume

6

fYear

1998

fDate

29 Oct-1 Nov 1998

Firstpage

2970

Abstract

The influence of bioelectret collagen on Chinese hamster ovary (CHO) cell growth in vitro is studied. Bioelectret collagen is prepared by a corona charging technique using a specifically designed setup. Cells in different phases of cell cycle are synchronized with the method of serum-starvation. Cell proliferation is determined by MIT assay. It is shown that bioelectret collagen can remarkably stimulate cell adhesion and proliferation. However, only cells in S phase are sensitive to the bioelectret stimulus among phases of G₀/G₁, S and G₂/M of the cell cycle. These results indicate that the bioelectret may influence cell growth via the modulation of both the cell adhesion and the cell cycle

Keywords

adhesion; bioelectric phenomena; biological techniques; biomedical materials; cellular biophysics; electrets; proteins; surface charging; Chinese hamster ovary cell; S phase cells; bioelectret collagen influence; bioelectret stimulus; cell adhesion; cell growth in vitro; cell proliferation; charge decay; corona charging; serum-starvation; Adhesives; Biological materials; Biomedical materials; Cells (biology); Coatings; Corona; Dielectric materials; Electrets; In vitro; Polarization;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1998. Proceedings of the 20th Annual International Conference of the IEEE

Conference_Location

Hong Kong

ISSN

1094-687X

Print_ISBN

0-7803-5164-9

Type

conf

DOI

10.1109/IEMBS.1998.746113

Filename

746113