3D single molecule tracking and superresolution microscopy using multifocal plane microscopy

Author

Ram, Sripad ; Ward, Elizabeth Sally ; Ober, Raimund J.

Author_Institution

Dept. of Immunology, Univ. of Texas Southwestern Med. Center, Dallas, TX, USA

fYear

2012

fDate

2-5 May 2012

Firstpage

914

Lastpage

915

Abstract

The study of cellular processes in three-dimensions is severely limited by the lack of imaging methodologies that allow for fast 3D tracking of cellular events and 3D superresolution imaging of sub-cellular structures. We have developed a 3D imaging modality, multifocal plane microscopy (MUM), that provides a powerful approach for 3D single molecule tracking and 3D superresolution microscopy. Here we review the technical challenges associated with 3D single molecule localization that is fundamental to both 3D tracking and 3D superresolution, and discuss how MUM overcomes these problems.

Keywords

biological techniques; cellular biophysics; maximum likelihood estimation; molecular biophysics; object tracking; optical microscopy; 3D single molecule localization; 3D single molecule tracking; 3D superresolution imaging; cellular processes; imaging modality; maximum likelihood estimation; multifocal plane microscopy; subcellular structures; superresolution microscopy; Accuracy; Image resolution; Microscopy; Optical microscopy; Optical variables measurement; Random access memory; 2D/3D localization accuracy; Cramer-Rao lower bound; Fisher information matrix; maximum likelihood estimator; resolution limits;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging (ISBI), 2012 9th IEEE International Symposium on

Conference_Location

Barcelona

ISSN

1945-7928

Print_ISBN

978-1-4577-1857-1

Type

conf

DOI

10.1109/ISBI.2012.6235702

Filename

6235702