Using approximation functions in analysis of trehaloze intracellular concentration

The study object is to evaluate in which measure the supplementation of yeast inoculums with linoleic acid succeed to minimize the negative effect of the thermal shock stress factor on the one side, and the subduing to repetitive running of fermentation cycles, on the other side. The study proposes to find the most efficient way to grow the intracellular trehaloze content through beer yeast suspending into trehaloze solutions by different concentrations, at different thermo-stating temperatures and in different contact times, taking into account that this technique allows the passive transfer of exogenetic trehaloze inside the cells both at a new propagated cell population, and at cells resulted from an industrial inoculums.