FEA for steady-state thermal performance of PCR thermal-cycler based on thermoelectric cooler

Author

Huang, Jing ; Chen, Zhangwei ; Hu, Kewei

Author_Institution

State Key Lab. of Fluid Power Transm. & Control, Zhejiang Univ., Hangzhou, China

Volume

2

fYear

2011

fDate

15-17 Oct. 2011

Firstpage

1097

Lastpage

1100

Abstract

Polymerase chain reaction (PCR) technology is a method for amplifying a specific target DNA sequence in vitro; thermoelectric cooler (TEC) is used in thermal-cycler system to implement periodic heating and cooling. Finite element models of multi-stage TEC and PCR thermal-cycler system based on TEC were established with ANSYS Workbench 12.0 thermal-electric coupled-field analysis elements. The steady-state thermal performances of TEC XLT2389 and thermal-cycler system with different driving currents were analyzed by this model. The simulation results indicated that the system steady-state temperature distribution was asymmetry for the energy loss on the sample block side surface; the system should work on a preferable driving current to achieve the optimal cooling ability.

Keywords

DNA; biomedical engineering; finite element analysis; molecular biophysics; thermoelectric cooling; ANSYS Workbench 12.0; DNA sequence; PCR technology; PCR thermal cycler; TEC XLT2389 and; cooling; finite element model; multistage TEC; periodic heating; polymerase chain reaction; steady state thermal performance; thermoelectric cooler; Analytical models; Cooling; DNA; Finite element methods; Heating; Steady-state; Thermal analysis; PCR; finite elment analysis; thermal-cycler; thermoelectric cooler;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2011 4th International Conference on

Conference_Location

Shanghai

Print_ISBN

978-1-4244-9351-7

Type

conf

DOI

10.1109/BMEI.2011.6098422

Filename

6098422