Title :
A new swappable fluidic module for applications of capillary convective polymerase chain reaction
Author :
Hsieh, Yi-Fan ; Yang, An-Shik ; Chen, Chia-Wei ; Liao, Shao-Kai ; Tseng, Li-Yu ; Chen, Ping-Hei
Author_Institution :
Dept. of Mech. Eng., Nat. Taiwan Univ., Taipei, Taiwan
Abstract :
A new swappable fluidic module (SFM) is demonstrated in this research. We designed and fabricated two basic modular fluidic components including pumps and mixers, which can be easily swapped and integrated into a variety of modular devices for ready assembly of a fully-portable, disposable fluidic system. An integrated SFM utilizes finger-driven electricity free pumps to transport the fluids. Using the swirling mechanism, the vortex mixer, simultaneously actuated by two pumps, has established a mixing index of 93% in a one-shot mixing event. The assembled SFM, working as a sample loader, was serially jointed a DNA application zone to establish a capillary convective polymerase chain reaction (ccPCR) test kit for performing the experiments of rapid DNA amplification. The successful results indicate the amplified DNA sequences from Hepatitis B Virus (HBV) in 30 min using ccPCR. Besides, the current SFM in conjunction with ccPCR show great potential for many bio-applications.
Keywords :
DNA; biochemistry; biological techniques; cellular biophysics; microorganisms; molecular biophysics; molecular configurations; HBV; Hepatitis B Virus; SFM; amplified DNA sequences; bioapplications; capillary convective polymerase chain reaction test kit; disposable fluidic system; finger-driven electricity free pumps; mixing index; modular devices; modular fluidic components; one-shot mixing event; rapid DNA amplification; swappable fluidic module; time 30 min; vortex mixer; DNA; Electron tubes; Fluids; Heating; Mixers; Polymers; Pumps;
Conference_Titel :
Automation Science and Engineering (CASE), 2012 IEEE International Conference on
Conference_Location :
Seoul
Print_ISBN :
978-1-4673-0429-0
DOI :
10.1109/CoASE.2012.6386450