Biological image segmentation based on object pigmentation

A methodology for segmenting biological images based on object pigmentation is proposed. The object of interest is extracted based on MacAdam´s just noticeable difference (see J. Opti. Soc. Am., vol.32, p.247-74, 1942). The method combines pictorial and spectral information, into a single valued total color difference. It has two important properties: accuracy based on human color observation and easy implementation. Experiments show that the proposed method is relatively insensitive to nonuniform illumination. The authors briefly review the MacAdam´s ellipses in colorimetry and derive a total color difference based on the MacAdam´s ellipses